Eulicin inhibits human immunodeficiency virus infection and replication.

munication, we investigated its effect on humanimmunodeficiency virus (HIV) infection and replication. The experiment analyzing effects on primarily infection was performed as follows. H9 cells4) were pretreated with serially diluted eulicin at 37°C for 60 minutes and infected with HIV-1 IIIB at 2x 103 TCID50. The cells were incubated for an additional 60 minutes to permit adsorption of viral particles to cells, then diluted with fresh media 1 : 10 and cultured in a 96 well plate. On day 6, the culture was harvested for reverse transcriptase (RT) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazoli-